Plant Diversity ›› 2009, Vol. 31 ›› Issue (02): 166-172.DOI: 10.3724 SP.J.1143.2009.08196
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XIAO Qiang1 , 2 , LIN Xiao-Mei2 , WU Fei-Hua2 , CHEN Juan2 , ZHENG Hai-Lei2
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肖 强1 , 2 , 林晓梅2 , 吴飞华2 , 陈 娟2 , 郑海雷2
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Abstract: The aim of the present paper was to find out a simple and easy- to-go method to observe the stomatal movement of a mangrove plant Aegiceras corniculatum . The new method was used to study the following areas, inculing the effect of exogenous nitric oxide (NO) on the leaf stomatal movement under different concentrations and different treated time , theeffect of Ca2+ in the regulation of stomatal movement induced by exogenous NO, the interactional effect between NO and H2O2 in the regulationb of stomatal movement. The results suggested that the oxhide pastern print was the best way to observe stomatal movement of A. corniculatum compared with the blender method and the nail enamel print method. The stomatalclosure induced by NO was associated with Ca2+ in dose dependent manner. It was also proved that a rapid stomatal closure could be co- induced by NO and H2O2 in A. corniculatum guard cells .
Key words: Nitric oxide
摘要： 探索一种用材简单、操作方便、真实性强的观察红树植物桐花树叶片气孔的制片技术, 并利用该技术研究不同浓度、不同处理时间的一氧化氮(NO) 供体硝普钠( sodium nitroprusside, SNP) 对桐花树气孔开闭的影响, 探讨了NO 调控的气孔运动与外源Ca2 + 的关系以及NO 与H2O2 在调节气孔运动过程中的关系。结果表明: 在搅碎法、指甲油印迹法、牛皮胶印迹法三种观察气孔方法中, 牛皮胶印迹法是观察气孔开度变化的最佳方法。NO 能够诱导桐花树气孔快速关闭, 且表现出明显的时间效应与浓度效应。NO 导致的气孔关闭与Ca2+ 的参与有密切关系, NO 与H2O2 存在明显的协同效应, 可以促进气孔关闭。
关键词: 一氧化氮, 桐花树, 气孔, 钙, H2O2
CLC Number:
Q 945. 1
XIAO Qiang , , LIN Xiao-Mei , WU Fei-Hua , CHEN Juan , ZHENG Hai-Lei. Effect of Exogenous Nitric Oxide Donor Sodium Nitroprusside on Stomatal Movement in Leaves of Mangrove Plant Aegiceras corniculatum[J]. Plant Diversity, 2009, 31(02): 166-172.
肖 强1 , 2 , 林晓梅2 , 吴飞华2 , 陈 娟2 , 郑海雷2 . 外源一氧化氮供体硝普钠对红树植物桐花树气孔运动的调控效应[J]. Plant Diversity, 2009, 31(02): 166-172.
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URL: https://www.integrativebiology.ac.cn/pd/EN/10.3724 SP.J.1143.2009.08196
https://www.integrativebiology.ac.cn/pd/EN/Y2009/V31/I02/166
